Coding

Part:BBa_K1602013

Designed by: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun   Group: iGEM15_TU_Darmstadt   (2015-09-14)

Aldehyde reductase - YqhD

YqhD encodes for a NADP+ dependent aldehyde reductase from E. coli. The enzyme is able to reduce a broad range of small aldehyde substrates like glycoaldehyde to ethyleneglycol.
https://static.igem.org/mediawiki/parts/8/81/BBa_K1602013_image_1.png

Characteristics


Molecular Weight 42097.02
Residues 387
Charge -3.5
Isoelectric Point 6.0466
A280 Molar Extinction Coefficients 42400 (reduced) no intramolecular cystine bridges possible
[Data taken from PEPSTATS]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 424
    Illegal AgeI site found at 618
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1081
[edit]
Categories
Parameters
None